To identify a suitable method of decalcification for mandible bone

• Research type

  Research Study

• Full title

  Identifing a suitable method for decalcification for mandible bones using three different decalcifying agents and specimen radiography, to preserve optimum tissue morphology and to improve the turnaround time for decalcification.

• IRAS ID

  187944

• Contact name

  Anthony Warford

• Sponsor organisation

  The Doctors Laboratory(TDL) in partnership with London Northwest Healthcare Trust

• Duration of Study in the UK

  0 years, 5 months, 26 days

• Research summary

  Decalcification is pre-analytical process in histology examination. It involves softening of bony tissue by placing in weak acids to make them compatible for section cutting. It's very essential to choose appropriate decalcification method to determine the optimal end point test of decalcification, thus to preserve the microscopic cellular structure for diagnostic purpose. Weak mineral acids are used remove Calcium salts from the bone and soften the bone texture and using the radiography (X-ray) can reveal the presence of Calcium in X-ray image. There are several short-comes in decalcification of mandible using current protocol such as not meeting the turnaround time( taking longer period for decalcification), difficulties in section cutting, poor quality of the mandible section for diagnosis.Therefore we would like esablish a better decalcification method using 3 different decalcification solutions and 2 different end point test to improve the turnaround time of decalcification of mandible and to gain better quality sections with preserving cellular morphology for diagnostic purpose.

• REC name

  East Midlands - Nottingham 2 Research Ethics Committee

• REC reference

  15/EM/0409

• Date of REC Opinion

  25 Aug 2015

• REC opinion

  Favourable Opinion