The use of Immunocytochemistry within cervical cytology - Version 1.
Research type
Research Study
Full title
The use of Immunocytochemistry within cervical cytology to identify the origin of Hyperchromatic Crowded Groups (HCGs).
IRAS ID
130666
Contact name
Nazar Alsanjari
Contact email
Sponsor organisation
Basildon and Thurrock Hospital
Research summary
I intend to use Immunocytochemistry (ICC) (a common laboratory technique that uses antibodies to target specific protein antigens in a cell, and these bound antibodies can then be detected through staining techniques) on Liquid-Based cervical smear samples that contain abnormal Hyperchromatic Crowded Groups (HCGs) (crowded groups of dark staining cells, the origins of which are not easily identifiable microscopically). HCGs are a frequent cause of interpretational errors within cervical screening, as the dark staining and crowding can make the groups difficult to interpret. If an abnormal HCG is detected through screening, then it can either be of squamous or glandular origin. The management of the patient is different dependent upon whether the abnormal group is diagnosed as being of squamous origin, compared with a diagnosis of glandular origin. Therefore, it is important that the diagnosis given by the screener is as accurate as possible, and by using ICC upon these samples, it is hoped that the origin of the group can be accurately identified, thus allowing for better patient management.
REC name
North East - Newcastle & North Tyneside 1 Research Ethics Committee
REC reference
13/NE/0302
Date of REC Opinion
10 Oct 2013
REC opinion
Favourable Opinion