The role of microRNAs in Kidney Disease (version 1)
Research type
Research Study
Full title
A feasibility study of the role of microRNAs in proximal tubular epithelial cells in disease: implications for the aetiology of tubulointerstitial inflammation and scarring.
IRAS ID
111062
Contact name
Juan Mason
Contact email
Sponsor organisation
Portsmouth Hospitals NHS Trust
Research summary
Research question / hypothesis:
MicroRNA (miR) expression changes in diseased human renal proximal tubule cells.
Study Design:
Laboratory study of archival paraffin block renal biopsies
Study Participants:
Diabetic renal patients with and without a transplant who have had a renal biopsy
Planned Sample Size:
30
Follow-up duration:
none
Planned Study Period:
6 weeks
Primary Objective:
To measure the expression of miRs in healthy and diseased tissue.
Secondary Objectives:
1.To perform serial staining on healthy and diseased kidney tissue to optimise the identification and subsequent isolation of proximal tubular cells using laser capture microdissection (LCM)
2.To measure the relative expression of miRs in healthy kidney tissues at different time points to see whether there is miR degradation in older specimens.
3.To analyse miR targets in silico using candidate miRs identified from diseased tissue and experimental studies
Primary Endpoint:
A panel of miRs that may influence the development of fibrosis in the kidney will be defined.
Secondary Endpoints:
After validation of gene targets the data obtained from the proximal tubules of diseased tissue will be interrogated to assess whether the changes in miRs, or the identified changes to targets, are found more widely in disease and whether they correlate with clinical signs and symptoms.
Interventions:
none
Key laboratory techniques:
Laser Dissection Microdissection; Immunohistochemistry; qPCR; rtPCRREC name
East of England - Cambridge Central Research Ethics Committee
REC reference
13/EE/0435
Date of REC Opinion
6 Dec 2013
REC opinion
Further Information Favourable Opinion