The Orange Juice and Cardiovascular Disease Study
Research type
Research Study
Full title
The Orange Juice and Cardiovascular Disease Study
IRAS ID
155431
Contact name
Paul A Kroon
Contact email
Sponsor organisation
Quadram Institute Bioscience
Research summary
This study will compare the effect of blood orange juice consumed daily for 4 weeks with that of a standard (blonde) orange juice consumed daily for 4 weeks, on markers of cardiovascular disease on an at risk group.
Forty two participants aged between 25 and 84 years will be recruited based on their waist measurement. Men will have a waist measurement = 102cm and women, a waist measurement of = 88cm.
This is a randomized 2-way cross over study with each participant drinking 500mL of either blood or standard (blonde) orange juice for 28 days, with a 21 day “wash out” period prior to drinking the other juice for another 28 days. The order in which the participant drinks the juice will be decided at random.
Prior to the start of the 1st phase of the intervention there will be a 2 week ”run in period” where participants will be asked to reduce their consumption of foods rich in anthocyanins. This low anthocyanin diet will be continued throughout the intervention until 24 hours before each of the four study days, where the participants will be asked to AVOID ALL foods on the list.
Blood samples will be collected for the production of plasma for the analysis of anthocyanins, their metabolites as well a CVD risk markers. Transcriptomics will be carried out on samples from peripheral blood mononuclear cells (PBMCs) derived from whole blood. Urine samples will be collected and urinary excretion of anthocyanins and their metabolites will be analysed. Arterial stiffness will also be assessed using pulse wave velocity and associated outputs. Measurements using the TANITA body analysr machine will also be performed which include fat mass, muscle mass, fat percentage, fat free mass, total body water, bone mass, metabolic age, basal metabolic rate, visceral fat rating, degree of obesity and BMI.
REC name
East of England - Cambridgeshire and Hertfordshire Research Ethics Committee
REC reference
14/EE/0219
Date of REC Opinion
24 Jun 2014
REC opinion
Further Information Favourable Opinion