Paediatric surfactant biology - Part II- SARPS II

• Research type

  Research Study

• Full title

  Pulmonary surfactant biology in ventilated children on a paediatric intensive care unit with acute hypoxic respiratory failure: the effects of artificial surfactant therapy - a pilot study-Part II (SARPS II)

• IRAS ID

  69617

• Contact name

  Michael J Marsh

• Sponsor organisation

  Southampton University Hospitals NHS Trust

• Eudract number

  2010-023668-42

• ISRCTN Number

  N/A

• Clinicaltrials.gov Identifier

  N/A

• Research summary

  We have previously demonstrated a significant disruption of the normal profile of phospholipids found in bronchoalveolar lavage (lung) fluid taken from children with respiratory failure. These molecules are the major constituen's of surfactant, a substance that is secreted by cells lining the air sacks of healthy children and adults. Normally surfactant acts as a detergent, lowering forces that promote lung collapse and allows the air sacks to remain open and able to participate in gas exchange (oxygen in and carbon dioxide out). In respiratory failure changes in'surfactant composition greatly reduce its detergent activity promoting lung collapse and impaired gas exchange. What we do not know is whether injury reduces the rate of surfactant production or increases the rate of breakdown. Deuteriated choline CHOLINE CHLORIDE(TRIMETHYL-D9) is a stable isotope of naturally occurring choline that we have used in part I of this study to safely and successfully label surfactant and study its metabolism in children with respiratory failure. The results are still being analysed but the initial data have validated the utility of this technique in this clinical setting. In other works the signal produced by the choline label is rapidly detectable and easily interpreted.This second stage of the study will ask two further questions in children with acute hypoxic respiratory failure:d) What is the effect of administration of a therapeutic dose of exogenous surfactant on the kinetics of incorporation of methyl-D9 choline into pulmonary surfactant?c) Can the initial rate of incorporation of methyl-D9 choline into pulmonary surfactant PC be used a predictor of the efficacy of administration of therapeutic doses of exogenous surfactant?

• REC name

  South Central - Oxford B Research Ethics Committee

• REC reference

  10/H0605/81

• Date of REC Opinion

  25 Jan 2011

• REC opinion

  Further Information Favourable Opinion