Metabolic profiling of weight-loss in overweight and obese children-V1
Research type
Research Study
Full title
The effect of a structured weight-loss programme on the metabolic profiles of obese and overweight children.
IRAS ID
182338
Contact name
Elaine Holmes
Contact email
Sponsor organisation
Imperial College London
Duration of Study in the UK
12 years, 0 months, 1 days
Research summary
TITLE: Characterising the effects of a structured diet and exercise programme on the metabolic profile of obese and overweight children as they lose weight.
AIMS: To identify changes in chemicals in the urine and stool associated with weight-loss in overweight and obese children following a diet and exercise camp.
DESIGN: Participants who are attending the MORElife summer camp in Leeds for weight loss will be approached to provide a urine and stool sample prior to commencing their time at the camp. They will then as part of the camp undergo a 2-5 week programme of diet, ecercise, education and psychosocial support. Further samples will be taken on the middle and final day of camp. These will be analysed using metabolic profiling techniques such as nuclear magnetic resonance (NMR) and Mass Spectroscopy to compare how the metabolites (chemicals) in their urine and stool gut bacteria change with weight loss.
POPULATION: Healthy overweight or obese boys and girls age 8-17 who have signed up to take part in the MORElife weight loss camp.
ELIGIBILITY: All children age 8-17 attending the MORElife 2015 Summer Camp.
DURATION: 5 weeks (summer camp) followed by a one off follow-up sample - up to 12 months after camp so total of 1 year for complete study.
OUTCOME MEASURES: The primary outcome measure and end point of this study is the effect of the weight loss on the metabolic profile and gut bacteria in children.
These effects will be assessed through analysis of urine and stool samples taken before, during and after weight loss using a combination of untargeted and targeted spectroscopic assays. This includes global profiling via NMR spectroscopy and ultra-performance liquid-chromatography (UPLC)-MS.
Secondary outcome measures:
• Weight loss
• Duplicate meal: analysis of food contents using the same above techniques to compare chemical contents in what the children eat with those in their urine/stool samples.REC name
South West - Cornwall & Plymouth Research Ethics Committee
REC reference
15/SW/0162
Date of REC Opinion
7 Jul 2015
REC opinion
Further Information Favourable Opinion