Human peripheral blood lymphocytes for therapeutic antibody screening
Research type
Research Study
Full title
Human peripheral blood lymphocytes for therapeutic antibody screening against T-cell co-receptors.
IRAS ID
196444
Contact name
Kevin J Roberts
Contact email
Sponsor organisation
VHsquared Ltd
Clinicaltrials.gov Identifier
7286540 , VHsquared Ltd registered in England & Wales
Duration of Study in the UK
2 years, 11 months, 18 days
Research summary
The VHsquared research team at the Wellcome Trust Sanger Institute (WTSI) has developed methods for the production of antibody fragments (functional antibody domains) that retain the antigen binding specificity and potency of conventional antibodies. The aim of the current project is to isolate single domain antibodies that bind to specific cell-surface receptors involved in the regulation of human T cells.
A library of domain antibodies has already been prepared. The aims of the current project will be to isolate from the library those domain antibodies that bind to human T cells and to investigate the properties of individual antibodies in terms of T cell receptor specificity, receptor binding characteristics and modulation of T cell functions. To this end, and using simple and well established procedures, we will need to isolate human T cells from whole human blood (or buffy coat packs) for use in the different cell-based research assays systems. Non-human or repeat-cultured human T cell lines often do not reflect the properties of primary human cells isolated directly from blood. Consequently, for this project, we will require primary human T cells, direct from blood. Thus we are seeking ethical permission to use anonymised bloods (or blood derived cells) acquired by the NHS Blood and Transplant service or commercial suppliers for the purpose of this research.REC name
East Midlands - Nottingham 2 Research Ethics Committee
REC reference
15/EM/0573
Date of REC Opinion
17 Dec 2015
REC opinion
Favourable Opinion