Genetic Screening of Anonymised Stem Cell Donor DNA Samples V1.0
Research type
Research Study
Full title
Genetic Screening and Gene Variance Analysis of Anonymised Stem Cell Donor Peripheral Blood DNA samples pre and post G-CSF Administration using Next Generation Sequencing (NGS) techniques
IRAS ID
198259
Contact name
Elisabeth Nacheva
Contact email
Sponsor organisation
Anthony Nolan
Duration of Study in the UK
1 years, 0 months, 1 days
Research summary
Our group recently conducted a Clinical trial entitled “Impact of Stem Cell Donation on Unrelated Donors” Rec Ref: 08/H0720/2. To investigate this question we used Fluorescence in situ hybridization (FISH) to look for changes at three chromosome regions (before and after donors received G-CSF that are commonly seen in large numbers of cells in patients with leukaemia. We did not observe any such chromosomal changes; instead we detected single cells with variable changes, which are the result of cell aging (dying) and renewal process. \n\nAll donors also consented to further investigations in case leukemic type chromosome changes were observed. We stored DNA from the donor’s peripheral blood samples for this purpose but the had no abnormal findings to analyse. However, we can now screen the stored DNA samples at a much higher resolution for changes occurring at single DNA level using the new powerful Next Generation Sequencing (NGS) technology. We expect to extend the proof of absence of detrimental effect of G-SCF administration at gene level.\n\nWe propose to use the Illumina NGS TruSight myeloid panel to screen for mutations in 54 genes known to be altered in leukaemia. Donor DNA samples pre and post GCSF administration will be compared. All work will be carried out in the OncoGenomics laboratory at Health Service Laboratories Analytics LLP under the supervision of Dr E Nacheva , Head of the Lab and Reader at UCL. We expect the study will be completed within 12 months.
REC name
West of Scotland REC 5
REC reference
16/WS/0036
Date of REC Opinion
5 Feb 2016
REC opinion
Further Information Favourable Opinion