Functional genomics of knee Osteoarthritis Version 2
Research type
Research Study
Full title
(The use of primary tissue to investigate) Functional Genomics of Knee Osteoarthritis
IRAS ID
328269
Contact name
Gisela Orozco
Contact email
Sponsor organisation
University of Manchester
Duration of Study in the UK
5 years, 0 months, 0 days
Research summary
Background
Many of the genes involved in osteoarthritis (OA) have now been identified but the DNA changes and its role in OA development is not well understood. DNA is deoxyribonucleic acid, and it directs our body to make proteins. RNA, ribonucleic acids are intermediate messengers, also essential for protein production. RNA sequencing provides information about the presence and quantity of these messengers. Integrating genetic information with datasets including gene expression levels and protein levels, increase our understanding of the pathogenesis of OA.
Why is it important?
Osteoarthritis is the most common joint disease worldwide, affecting approximately 10% of men and 18% of women over 60. It is a whole joint disease impacting all joint tissues causing pain and disability. Certain forms of OA are known to have a strong genetic component.
The questions it will answer……
This data will provide insights into the mechanisms involved in the development of OA.
Potential Benefits
Improve the understanding of the genetic mechanisms in OA using gene expression data, proteomic data, and epigenetic data.
Study Design
Study participants are recruited from patients undergoing knee arthroscopy or total knee replacement. Discard tissue from these procedures will be transported to the University of Manchester for genomic analyses. A blood sample will also be obtained for genetic and proteomic analysis. Study participants who are not affected by osteoarthritis but undergoing routine diagnostic or therapeutic knee arthroscopy will also be recruited to this study to act as a control group. Further sequencing of synthetic libraries will be performed at the Genomics Core Facility at the Helmholtz Zentrum München, Germany, or another service provider such as Novogene. Demographic information, imaging results and standard of care patient reported outcome measures on pain and function will be used to support the interpretation of the genomic analyses.Funding the Research
This study will initially be funded by a grant held by Principal Investigator, Dr Gisela Orozco at The University of Manchester. This includes Wellcome Trust (Senior Fellowship in Basic Biomedical Science), Arthritis Research UK Centre of Excellence in Genetics and Genomics and Manchester NIHR Biomedical Research Centre: Musculoskeletal Theme
Where it will be Recruiting
Study participants will be recruited at the Manchester University Foundation Trust, elective orthopaedic clinics by orthopaedic consultants named on the delegation log for this study.REC name
North West - Greater Manchester West Research Ethics Committee
REC reference
23/NW/0136
Date of REC Opinion
23 Jun 2023
REC opinion
Further Information Favourable Opinion