Development of haematological analysis techniques
Research type
Research Study
Full title
Development of haematological analysis techniques
IRAS ID
234136
Contact name
Chris Burrows
Contact email
Sponsor organisation
Entia Ltd
Duration of Study in the UK
3 years, 0 months, 0 days
Research summary
Aim
In this project we aim to calibrate and test the measurement techniques under development against gold-standard technologies in order to understand the viability of the techniques. In order to conduct this investigation samples shall be acquired from healthy individuals.
Study DesignOne sample of blood shall be taken from participants. The means of acquiring the sample shall be through venepuncture or a finger prick. Aseptic venepuncture will be conducted by a trained clinician. The blood shall be taken directly into a vial containing an anticoagulant. No identifiable data shall be associated with the samples. The participants will not be involved following this. The whole blood shall be treated as follows.
Part of each sample shall be analysed by an automated analysis device (i.e. HORIBA ABX Pentra 60) to characterise the samples haematological parameters (e.g. Hgb, Hct, MCV, WBC, RBC, RDW, MCHC, MCH).
The sample or a part of the sample may be centrifuged (5000 rpm for 5 minutes at room temperature) and separated into constituents of blood cells and serum. They may then be recombined to create samples with artificially high or low blood constituents (red blood cells, white blood cells etc.).
A portion of the original sample shall be placed in a specially designed cuvette and centrifuged (0-10,000 rpm for 1-6 minutes at room temperature) whilst being analysed by optical devices.
The results given by the optical analysis techniques shall be correlated and compared to the results from the automated analyser.REC name
London - Surrey Borders Research Ethics Committee
REC reference
18/LO/2165
Date of REC Opinion
12 Dec 2018
REC opinion
Favourable Opinion