CCN 1-3 expression by HUVEC in uremic medium

• Research type

  Research Study

• Full title

  CCN 1-3 protein expression by Human Umbilical Vein Endothelial Cells on exposure to uremic serum from End Stage Kidney Disease patients

• IRAS ID

  212471

• Contact name

  NIhil Chitalia

• Contact email

  nihil.chitalia@nhs.net

• Sponsor organisation

  Darent Valley Hospital NHS trust

• Duration of Study in the UK

  0 years, 3 months, 30 days

• Research summary

  Comprehensive Clinical Network proteins (CCN1-6) are a family of key regulatory signalling proteins performing important biological functions that regulate extracellular matrix (ECM) formation.

  We have noticed increased ECM formation in the tunica intima of muscular arteries obtained patients with chronic kidney disease (CKD). This ECM is responsible for clinical diseases such as hypertension and atherosclerosis in CKD patients. We propose that vascular endothelial cells secrete CCN family of proteins 1-3, which are dysregulated when exposed to uremia.

  This hypothesis will be tested in-vitro using Human umbilical vein endothelial cells (HUVEC) exposed to uremic serum from 10 patients on dialysis and 10 healthy controls without renal disease.

• REC name

  East of England - Cambridge South Research Ethics Committee

• REC reference

  16/EE/0400

• Date of REC Opinion

  28 Nov 2016

• REC opinion

  Further Information Favourable Opinion