Association between periodontal disease and diabetic foot ulcer
Research type
Research Study
Full title
Association between periodontal disease and diabetic foot ulcer using the SIFT-MS and the electronic nose
IRAS ID
242662
Contact name
Saliha Saad
Contact email
Sponsor organisation
University of the Westof england
Duration of Study in the UK
0 years, 11 months, 28 days
Research summary
The human body is subjected to microbial colonisation and external sites are colonised by characteristic groups of microorganisms in health and disease. These produce volatile organic compounds (VOC) and volatile sulfur compounds (VSC) which give rise to body odor. Headspace gases forming above the sites in question can be sampled and measured using instruments (gas chromatography, mass spectroscopy such as SIFT-MS; portable electronic nose such as NeOse) and microbial sampling of key different sites (e.g. mouth, toe webs and foot) can be used to determine population numbers and types of microbes present. In addition, microbial samples can be used to inoculate a laboratory "biofilm model" so that mouth and foot condition/odour can be replicated in vitro. Thus, associations (relationships) may be seen between odour compounds (VSC/VOC) and quantity or type of microorganisms present. Moreover, different VOC profiles may give us an insight into the biochemical processes at a specific body site identifying the different stages in the evolution of the disease when present. Identifying the species and numbers of microbes present at a particular body site, and/or using the in vitro model allow the investigators to look for strong association or relationships to be seen between microbial numbers or types at a particular sample site and VSC/VOC concentrations and subsequent body odor. The research is of interest in order to find the best approach to diagnose, monitor and control body odor of microbial origin, or link the profile of volatile organic compounds (VOC) detected, to the different types of oral and skin conditions (periodontal disease and diabetic foot ulcer). Meanwhile, the in vitro model may also be used to screen antimicrobial products (e.g. inhibitory compounds or biocides) that may be best used to combat the site-specific infections if used in vivo.
REC name
South Central - Berkshire B Research Ethics Committee
REC reference
18/SC/0167
Date of REC Opinion
29 Mar 2018
REC opinion
Further Information Favourable Opinion