Analysis of PBMC Responses to Test Samples v. 1
Research type
Research Study
Full title
Analysis of proliferation and cytokine secretion of peripheral blood mononuclear cells in response to stimulation with test samples
IRAS ID
170600
Contact name
Mark Fogg
Contact email
Duration of Study in the UK
1 years, 0 months, 16 days
Research summary
White blood cells (peripheral blood mononuclear cells, PBMC) will be isolated from waste products of routine blood donations (apheresis cones or buffy coats) provided by healthy adults to NHS Blood and Transplant (NHSBT). As part of the donation process, blood donors are asked to consent to the possibility that part, or all, of their donation may be used for research. PBMC will also be isolated from commercial blood sources (buffy coats or whole blood) where the supplier has obtained similar consent from donors. All blood products are supplied to Antitope within 36 hours of blood draw. Antitope will use the PBMC to test for immune responses to drugs that are in development to determine their potential to produce unwanted side effects or be ineffective when administered to patients, thus helping to ensure that potentially unsafe or ineffective medicines are not given to patients. This assessment will include measuring any increase in the rate of cell division of specific cell types and the secretion of proteins which can stimulate the immune system. Antitope will also determine the human leukocyte antigens (HLA) present in each donor (tissue typing) to ensure that a broad representation of the general population is used in the study and to determine whether a particular HLA is associated with any response observed to the drugs being tested. Tissue typing will be undertaken by analysing DNA from the donor blood. Up to 100 donor samples will be used for the study.
REC name
Yorkshire & The Humber - Sheffield Research Ethics Committee
REC reference
14/YH/1313
Date of REC Opinion
19 Dec 2014
REC opinion
Favourable Opinion