1) Compare platelet activation using the Advia 2120 and flow cytometry.
Research type
Research Study
Full title
A comparison of platelet activation measured by the Advia 2120 and flow cytometry and its correlation with the clinical picture in patients with essential thrombocythaemia and idiopathic thrombocytopenia purpura.
IRAS ID
136742
Contact name
Michael Colborne
Contact email
Sponsor organisation
South Tees Hospitals NHS Foundation Trust
Duration of Study in the UK
1 years, 6 months, 31 days
Research summary
The purpose of this study is to measure platelet activity on patients with idiopathic thrombocytopenia (ITP) and essential thrombocythaemia (ES).
Patients with ITP generally have a low platelet count, whereas patients with ES have a raised platelet count. Both groups of patients can have bleeding and thrombotic (develop a blood clot) complications.
Platelets are small discoid blood cells that are made in the bone marrow. They have a lifespan of 7-10 days. In a normal resting state platelets require a trigger to become activated. Platelets are activated to help your blood to clot and they assist in wound healing.
This study will be looking at whether the platelets are activated in people with ITP and ES. Which means they are trying to stop a bleed or they are helping the body to heal.
Currently, platelet activity can be measured using an analyser called a Flow Cytometer. Using this analyser is time consuming and expensive.
Another analyser called Advia 2120 can also measure platelet activity and it is quicker and cheaper to use.
The Advia 2120 can assess platelet activation using a parameter called the mean platelet component (MPC), whereas the flow cytometer measures a molecule called CD62P which is found on activated platelets.
Blood from healthy volunteers will be used to generate a local reference range for the mean platelet component and CD62P. This reference range will give an indication of the degree of platelet activation present in persons who do not have idiopathic thrombocytopenia purpura or essential thrombocythaemia.
This study is to assess the two different methods to see if the Advia 2120 is just as good as the Flow Cytometer in measuring platelet activity.
REC name
North West - Liverpool Central Research Ethics Committee
REC reference
15/NW/0475
Date of REC Opinion
4 Jun 2015
REC opinion
Further Information Favourable Opinion